Reverse Slot Blot for the Verification of cDNAs Identified through Differential Display
نویسندگان
چکیده
منابع مشابه
Reverse slot blot for the verification of cDNAs identified through differential display.
Differential display, first described by Liang and Pardee (4), has become a powerful screening tool for the identification of cellor stage-specific gene expression. The technique relies on efficient reverse transcription of mRNA and subsequent amplification of this cDNA with as many random primer combinations as possible. RT-PCR products are separated on denaturing sequencing gels, visualized t...
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An accurate and streamlined approach to differential display (DD) band identification and verification is described. To minimize false positives, the strategy avoids the use of impure Northern blot probes obtained from PCR-amplified DD bands. To increase throughput, the cloning of DD bands is replaced by a gene-specific primer approach, and hybridization arrays are used in place of Northern blo...
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Better gel resolution and longer cDNAs increase the precision of differential display.
become a widely used method for the rapid identification of differentially expressed genes in a variety of eukaryotic systems (4). In the past three years, technical modifications have reduced the redundancy of anchored primers, decreased the number of reverse transcription reactions needed, increased reproducibility, reduced the incidence of false positives and increased the overall efficiency...
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assembly with dimensions of 14 x 17 in. can accommodate 12 high-density arrayed filters (8 x 12 cm) or 15 circular filters (82 mm diameter). The overall results produce a significant increase in the throughput of the number of filters that can be processed with consistent results and the absence of any cross contamination. Cleaning the netting assembly is easily accomplished by rinsing with wat...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2003
ISSN: 0736-6205,1940-9818
DOI: 10.2144/03342bm07